{"product_id":"9783527341337","title":"Super-Resolution Microscopy : A Practical Guide","description":"\u003cp\u003eThis unique book on super-resolution microscopy techniques presents comparative, in-depth analyses of the strengths and weaknesses of the individual approaches. It was written for non-experts who need to understand the principles of super-resolution or who wish to use recently commercialized instruments as well as for professionals who plan to realize novel microscopic devices. Explaining the practical requirements in terms of hardware, software and sample preparation, the book offers a wealth of hands-on tips and practical tricks to get a setup running, provides invaluable help and support for successful data acquisition and specific advice in the context of data analysis and visualization. Furthermore, it addresses a wide array of transdisciplinary fields of applications.\n\u003cbr\u003eThe author begins by outlining the joint efforts that have led to achieving super-resolution microscopy combining advances in single-molecule photo-physics, fluorophore design and fluorescent labeling, instrument design and software development. The following chapters depict and compare current main standard techniques such as structured illumination microscopy, single-molecule localization, stimulated emission depletion microscopy and multi-scale imaging including light-sheet and expansion microscopy. For each individual approach the experimental setups are introduced, the imaging protocols are provided and the various applications illustrated. The book concludes with a discussion of future challenges addressing issues of routine applications and further commercialization of the available methods.\n\u003cbr\u003eGuiding users in how to make choices for the design of their own experiments from scratch to promising application, this one-stop resource is intended for researchers in the applied sciences, from chemistry to biology and medicine to physics and engineering. Preface \n\u003cbr\u003eAbbreviations \n\u003cbr\u003e \n\u003cbr\u003eINTRODUCTION\n\u003cbr\u003eThe Classical Resolution Limit\n\u003cbr\u003eMethods to Circumvent the Classical Resolution Barrier in Fluorescence Microscopy\n\u003cbr\u003eImplementation of Super-Resolution Microscopy (SRM)\n\u003cbr\u003eContrast\n\u003cbr\u003eApplications to Study Nuclear DNA\n\u003cbr\u003eOther Applications\n\u003cbr\u003e \n\u003cbr\u003ePHYSICOCHEMICAL BACKGROUND\n\u003cbr\u003eMotivation\n\u003cbr\u003eLabeling\n\u003cbr\u003eTransitions of the Fluorophores\n\u003cbr\u003eSamples\n\u003cbr\u003e \n\u003cbr\u003eHARD- AND SOFTWARE\n\u003cbr\u003eHardware Requirements \n\u003cbr\u003eSoftware \n\u003cbr\u003eOpen Source and Best Practice \n\u003cbr\u003e \n\u003cbr\u003eSTRUCTURED ILLUMINATION AND IMAGE SCANNING MICROSCOPY\n\u003cbr\u003eAxially Structured Illumination Microscopy (aSIM) \n\u003cbr\u003eLaterally Structured Illumination Microscopy (SIM) \n\u003cbr\u003eImage Scanning Microscopy \n\u003cbr\u003eSuper-Resolution Using Rotating Coherent Scattering (ROCS) Microscopy \n\u003cbr\u003e \n\u003cbr\u003eLOCALIZATION MICROSCOPY\n\u003cbr\u003eOn the Principles of Localization Microscopy \n\u003cbr\u003ePALM\/STORM\/fPALM\/SPDM Approach \n\u003cbr\u003eImplementation of SMLM \n\u003cbr\u003eOn the Principles of Three-Dimensional SMLM \n\u003cbr\u003eReduction of Out-of-Focus Light \n\u003cbr\u003eHow to Build a Three-Dimensional SMLM \n\u003cbr\u003eHigh-Density Single Emitter Microscopy Methods: SOFI, 3B, SHRImP, etc.\n\u003cbr\u003eApproaches Towards Counting Molecules \n\u003cbr\u003eRequirements and Sample Preparation \n\u003cbr\u003eData Acquisition \n\u003cbr\u003eData Analysis \n\u003cbr\u003eTroubleshooting \n\u003cbr\u003eMeta Analysis Tailored for SMLM \n\u003cbr\u003eExample Applications \n\u003cbr\u003e \n\u003cbr\u003eSTIMULATED EMISSION DEPLETION MICROSCOPY (STED)\n\u003cbr\u003eOn the Principles of Stimulated Emission Depletion Microscopy \n\u003cbr\u003eImplementation of STED \n\u003cbr\u003eFluorescent Probes \n\u003cbr\u003eDye Combinations for Dual-Color STED \n\u003cbr\u003eRequirements and Sample Preparation \n\u003cbr\u003eData Acquisition\n\u003cbr\u003eData Analysis and Visualization \n\u003cbr\u003eExample Applications \n\u003cbr\u003eConclusion \n\u003cbr\u003e \n\u003cbr\u003eMULTI-SCALE IMAGING\n\u003cbr\u003eLight-Sheet Fluorescence Microscopy (LSFM) \n\u003cbr\u003eOptical Projection Tomography (OPT) \n\u003cbr\u003eExpansion Microscopy (ExM) and Sample Clearing \n\u003cbr\u003eAlternative Approaches \n\u003cbr\u003e \u003c\/p\u003e\n\n\u003cp\u003eDISCUSSION\n\u003cbr\u003eFuture Challenges\n\u003cbr\u003eCommercialization of Super-Resolution Microscopes \n\u003cbr\u003eConcluding Remarks \n\u003cbr\u003e \n\u003cbr\u003eIndex\n\u003cbr\u003e Udo Birk studied physics and mathematics in Canada and Germany and obtained a PhD in applied physics from the University of Heidelberg (Germany) for his work on tissue spectrometry and spatially modulated illumination microscopy. He was a Marie-Curie Fellow at King's College London (UK) and at the Foundation of Research and Technology Hellas (Greece), where he worked on structured illumination microscopy and on optical projection tomography. Currently, he is deputy group leader at the Institute of Molecular Biology in Mainz (Germany), which equals a position of an associate professor, and his research focuses on the development and application of advanced optical imaging techniques.\u003c\/p\u003e","brand":"Wiley","offers":[{"title":"Default Title","offer_id":48807626637547,"sku":"00000_00000_00000_00000","price":247.12,"currency_code":"SGD","in_stock":true}],"url":"https:\/\/kinokuniya.com.sg\/products\/9783527341337","provider":"Books Kinokuniya Singapore","version":"1.0","type":"link"}