{"product_id":"9783527334643","title":"Analyzing Biomolecular Interactions by Mass Spectrometry (2015. XXII, 378 S. 100 SW-Abb., 40 Farbabb. 244 mm)","description":"\u003cp\u003e§16§This monograph reviews all relevant technologies based on mass spectrometry that are used to study or screen biological interactions in general. §Arranged in three parts, the text begins by reviewing techniques nowadays almost considered classical, such as affinity chromatography and ultrafiltration, as well as the latest techniques. The second part focusses on all MS-based methods for the study of interactions of proteins with all classes of biomolecules. Besides pull down-based approaches, this section also emphasizes the use of ion mobility MS, capture-compound approaches, chemical proteomics and interactomics. The third and final part discusses other important technologies frequently employed in interaction studies, such as biosensors and microarrays.§For pharmaceutical, analytical, protein, environmental and biochemists, as well as those working in pharmaceutical and analytical laboratories.§ §04§Preface§ §INTRODUCTION TO MASS SPECTROMETRY, A TUTORIAL§Introduction§Figures of Merit§Analyte Ionization§Mass Analyzer Building Blocks§Tandem Mass Spectrometry§Data Interpretation and Analytical Strategies§Conclusion and Perspectives§ §PART I: Direct MS Based Affinity Techniques§ §STUDYING PROTEIN-PROTEIN INTERACTIONS BY COMBINING NATIVE MASS SPECTROMETRY AND CHEMICAL CROSS-LINKING§Introduction§Protein Analysis by Mass Spectrometry§Native MS§Chemical Cross-Linking MS§Value of Combining Native MS with Chemical Cross-Linking MS§Regulating the Giant§Capturing Transient Interactions§An Integrative Approach for Obtaining Low-Resolution Structures of Native Protein Complexes§Future Directions§ §NATIVE MASS SPECTROMETRY APPROACHES USING ION MOBILITY-MASS SPECTROMETRY§Introduction§Sample Preparation§Electrospray Ionization§Mass Analyzers and Tandem MS Approaches§Ion Mobility §Data Processing§Chall §04§enges and Future Perspectives§ §PART II: LC-MS Based with Indirect Assays§ §METHODOLOGIES FOR EFFECT-DIRECTED ANALYSIS: ENVIRONMENTAL APPLICATIONS, FOOD ANALYSIS, AND DRUG DISCOVERY§Introduction§Principle of Traditional Effect-Directed Analysis§Sample Preparation§Fractionation for Bioassay Testing§Miscellaneous Approaches§Identification of Confirmation Process§Conclusion and Perspectives§ §MS BINDING ASSAYS§Introduction§MS Binding Assays - Strategy§Application of MS Binding Assays§Summary and Perspectives§ §METABOLIC PROFILING APPROACHES FOR THE IDENTIFICATION OF BIOACTIVE METABOLITES IN PLANTS§Introduction to Plant Metabolic Profiling§Sample Collection and Processing§Hyphenated Techniques§Mass Spectrometry§Mass Spectrometric Imaging§Data Analysis§Future Perspectives§ §ANTIVENOMICS: A PROTEOMICS TOOL FOR STUDYING THE IMMUNOREACTIVITY OF ANTIVENOMS§Introduction§Challenge of Fighting Human Enve §04§noming by Snakebites§Toolbox for Studying the Immunological Profile of Antivenoms§First-Generation Antivenomics§Snake Venomics§Second-Generation Antivenomics§Concluding Remarks§ §PART III: Direct Pre- and On-Column Coupled Techniques§ §FRONTAL AND ZONAL AFFINITY CHROMATOGRAPHY COUPLED TO MASS SPECTROMETRY§Introduction§Frontal Affinity Chromatography§Staircase Method§Simultaneous Frontal Analysis of a Complex Mixture§Multiprotein Stationary Phase§Zonal Chromatography§Nonlinear Chromatography§ §ONLINE AFFINITY ASSESSMENT AND IMMUNOAFFINITY SAMPLE PRETREATMENT IN CAPILLARY ELECTROPHORESIS-MASS SPECTROMETRY§Introduction§Capillary Electrophoresis§Affinity Capillary Electrophoresis§Immunoaffinity Capillary Electrophoresis§Capillary Electrophoresis-Mass Spectrometry§Application of ACE-MS§Applications of IA-CE-MS§Conclusions§ §LABEL-FREE BIOSENSOR AFFINITY ANALYSIS COUPLED TO MASS SPECTROMETRY§Introduct §04§ion to MS-Coupled Biosensor Platforms§Strategies for Coupling Label-Free Analysis with Mass Spectrometry§New Sensor and MS Platforms, Opportunities for Integration§ §PART IV: Direct Post Column Coupled Affinity Techniques§ §HIGH-RESOLUTION SCREENING: POST-COLUMN CONTINUOUS-FLOW BIOASSAYS§Introduction§The High-Resolution Screening Platform§Data Analysis§Conclusions and Perspectives§ §CONCLUSIONS§ §Index§\u003c\/p\u003e","brand":"WILEY-VCH","offers":[{"title":"Default Title","offer_id":48783944253675,"sku":"00000_00000_00000_00000","price":346.0,"currency_code":"SGD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0758\/4484\/5803\/files\/9783527334643-1.jpg?v=1781722691","url":"https:\/\/kinokuniya.com.sg\/zh\/products\/9783527334643","provider":"Books Kinokuniya Singapore","version":"1.0","type":"link"}