Behringer, Gertsenstein, Nagy, and Nagy edit this technical guide to murine embryonic research, with the new fourth edition including advances of the last ten years in gene sequencing, induced pluripotent stem cells, and imaging technique. The book leads with the history and relevance of mouse embryology and a summary of mouse development. Maintenance of a mouse colony, procurement and handling of pre- and post-implantation embryos, and surgical procedures are covered. Transgenic and stem cell techniques receive several chapters, followed by genotyping, cloning, assisted reproduction, cryopreservation of embryonic and gametic genetic stocks, and visualization techniques. The last chapter discusses instrumentation and lab requirements for micromanipulation. Detailed protocols are provided for many procedures, including specific volumes, concentrations, and reaction times. Appendices list buffers, reagents, stains, web resources, and hazard information. Annotation 2014 Book News, Inc., Portland, OR (booknews.com)
