§16§The third volume of "Advances in Forensic Haemogenetics" contains the th scientific contributions presented at the 13 Congress of the International Society for Forensic Haemogenetics, held on October 19-21, 1989 in New Orleans, USA. The conference was organized and chaired by Dr. Herbert Polesky from Minneapolis. He and the local organizing committee which consisted of our friends and colleagues (J. Soubrada, L.R.Bryant, Dale D.Dykes, Ch.Harrison, P.Newall and R. Walker) deserve the thanks of our Society for a very successful meeting. Herb Polesky has also contributed a great deal to the preparation of this book. The contributions to the conference covered all fields of forensic haemo genetics, but an outstanding highlight of this conference was the application ofDNA-polymorphisms to paternity and to the identification of stains. This included basic lectures on biostatistical approaches as well as on molecular biology and many new technical approaches to our general and special ai §16§ms. Forensic haemogenetics has now merged into a new discipline without having lost its original identity. On behalf of the Executive Committee of our Society I would like to extend my thanks to the authors of the articles contained in this book and to Springer-Verlag for having made such a quick publication possible. The volume should give the reader a picture of the state of the art and a survey of the most recent developments in the field of forensic and general haemo genetics. §04§1 DNA Polymorphisms.- 1.1 Biostatistics.- 1.2 Methodology, Data Bases.- 1.3 Practical Use.- 1.4 PCR.- 2 Conventional Markers.- 2.1 Alloantigens.- 2.2 Electrophoretic Polymorphisms.- 3 General Biostatistics.- 4 Varia. §04§Sex Determination Using Restriction Enzymes Digestion.- DNA Fingerprinting with Simple Repetitive Oligonucleotide Probes in Forensic Medicine.- Application of Simple Repeat Oligonucleotides for DNA Fingerprinting.- DNA Fingerprinting with M13 Probe.- Minisatellite DNA Probe MZ 1.3: Application in Paternity Testing and Estimate of the Number of Genetic Loci.- Non-isotopic DNA Fingerprint Analyses with the Minisatellite Probe MZ 1.3.- DNA Fingerprinting in Paternity Analyses.- A Review of the Cases Submitted for DNA Analysis in the FBI Laboratory.- 1.4 PCR.- A Method for the Analysis of PCR Amplification Products to Identify Base-Pair Substitution and VNTR Polymorphisms.- Analysis of PCR Products (pMCT118) by Polyacrylamide Gel Electrophoresis.- Effects of UV Damage on DNA Amplification by the Polymerase Chain Reaction.- Typing by Polymerase Chain Reaction Mediated Amplification of DNA Fragments in the HLA Class I Region and Direct DNA Sequencing.- Use of the Polymerase Chain Reacti §04§on for Typing GC Variants.- Amplification of Y-Chromosome-Specific Sequences in Biological Evidence.- 2 Conventional Markers.- 2.1 Alloantigens.- Differential Detection of A, B and H Antigens in Semen and Saliva Stains Detected by an ELISA Technique.- A Modified ELISA for the Detection of ABH Antigens in Bloodstains.- Mosaic Phenomenon of ABH and Lewis Antigen Expression in Minor Salivary Glands - An Immunohistochemical Demonstration of the Transferase Activity.- Different Expression of Blood Group A Antigen in the Secretory Cells of Salivary Glands from German and Japanese Nonsecretor Individuals.- Different Epitopes of ABH Antigens Exist in Red Cell and Body Fluids.- Production of Monoclonal Antibody to ABH-Carrying ?-2-Seminoglycoprotein for ABO-Grouping of Semen by Enzyme-Linked Immunosorbent Assay (ELISA).- Isolation and Identification of ABO Blood Group of Seminal Stains Mixed with Vaginal Secretion or Blood.- Histochemical Analysis of the Chemical Structure of Blood Group-R
