This book presents a historical overview of the development of vitrification techniques and discusses protocols for vitrifying various stages of cells and tissues. It covers standard and new cryopreservation techniques in detail, outlining those that have resulted in success, and providing recommended means for overcoming typically encountered problems. This new edition provides a much broader range of clinical application and data to demonstrate its contribution to the use of vitrified oocytes and embryos. The book also discusses new areas in the field of assisted reproductive technology such as oocyte banking, pre-implantation diagnostics at the blastocyst stage, and the burgeoning adoption of elective single embryo transfer.
