This laboratory manual provides basic techniques in yeast molecular genetics and cell biology, methodologies for functional genomics, approaches to computational analysis, and the use of databases for analysis of the budding yeast Saccharomyces cerevisiae. It outlines experimental procedures for culturing and genetically modifying yeast, characterizing cell structure and morphology, and probing the modifications and interactions of cellular constituents, as well as strategies for studying metabolomics, complex traits, and evolution in yeast, and methods to isolate and investigate new strains of yeast from the wild. It includes bioinformatics tools and resources and discussion of the historical evolution of laboratory yeast strains and classical genetics with Saccharomyces cerevisiae. Annotation 2016 Ringgold, Inc., Portland, OR (protoview.com)
