The study of polymerese chain reactions (PCRs) has proven valuable in providing both quantitative and qualitative data in all areas of microbiology. Mackay (Sir Albert Sakzewski Virus Research Centre, Australia) keeps the needs of both novices to the field and experienced users in this comprehensive handbook and reference in which he and his contributors ensure readers know how to make sure what appears to be happening really is happening. They describe the history of real-time PCR and fluorogenic chemistries, oligonucleotide design for applications, target preparation, standards and controls, quantification of microorganisms, the real role of multiplex real-time PCR in microbiology and in routine microbial diagnostics, challenges in acute respiratory tract infections and rapid detection of bioterror agents. The collection closes with a roundtable discussion featuring over 30 experts. Distributed in the US by ISBS. Annotation 2007 Book News, Inc., Portland, OR (booknews.com)
