§16§How can I use my HPLC/UHPLC equipment in an optimal way, where are the limitations of the technique? These questions are discussed in detail in the sequel of the successful "HPLC Expert" in twelve chapters written by experts in the respective fields. The topics encompass - complementary to the first volume - typical HPLC users' problems and questions such as gradient optimization and hyphenated techniques (LC-MS). An important key aspect of the book is UHPLC: For which analytical problem is it essential, what should be considered? Besides presentation of latest developments directly from the main manufacturers, also UHPLC users and independent service engineers impart their knowledge. Consistent with the target groups, the level is advanced, but the emphasis is on practical applications. §04§Introduction: When should I use my UHPLC as a UHPLC? § §PART 1: HARDWARE AND SOFTWARE, SEPARATION MODES, MATERIALS§Modern HPLC/UHPLC equipment§Requirements for the different parts of HPLC/UHPLC equipment§The column oven - a simple matter?§The problem of the extra column dispersion in an HPLC/UHPLC equipment§The gradient: Requirements, optimal operation, tricks and pitfalls§Hardware requirements for LC coupling with different mass spectrometers§2D-Chromatography - potentials and limitations §Selecting the right column materials in HPLC and UHPLC§Qality by Design in HPLC Method Development§ §PART 2: USE CASES AND TRENDS§What should the software be able to do, so that the hardware can be used to its full potential?§Aspects of modern HPLC - a user〓s experience report§Hints and recommendations for optimal use of HPLC equipment§Using UHPLC to solve the compound separation problem§Equipment review (Agilent, Shimadzu, ThermoScientific) §02§Stavros Kromidas studied biology and chemistry at the University of Saarbrücken, where he obtained his Ph.D. degree on the development of new chiral stationary phases for HPLC in 1983. After working for Waters GmbH for five years, he founded NOVIA GmbH, a provider of professional training and consulting in analytical chemistry, serving as the CEO until 2001. Since 2001 he works as an independent consultant for analytical chemistry, based in Saarbrücken (Germany). For more than 20 years he has regularly held lectures and training courses on HPLC, and has authored numerous articles and several books on various aspects of chromatography.
