§16§Now in full color for a more intuitive learning experience, this new edition of the long-selling reference also features a number of new developments in methodology and the application of enzyme kinetics.Starting with a description of ligand binding equilibria, the experienced author goes on to discuss simple and complex enzyme reactions in kinetic terms. Special cases such as membrane-bound and immobilized enzymes are considered, as is the influence of external conditions, such as temperature and pH value. The final part of the book then covers a range of widely used measurement methods and compares their performance and scope of application.With its unique mix of theory and practical advice, this is an invaluable aid for teaching as well as for experimental work. §04§PART I: Ligand BindingDiffusionInteraction between macromolecules and ligandsIdentical independent binding sitesNon-identical independent binding sitesIdentical interacting binding sites, cooperativityNon-identical interacting binding sitesPART II: Enzyme KineticsReaction OrderSteady-state kinetics and the Michaelis-Menten EquationReversible enzyme reactionsEnzyme inhibitionMulti-substrate reactionsRate equations of complex enzyme mechanismsAllosteric enzymespH and temperature dependenceIsotope exchange kinetics and isotope effectsRibozymesEnyzmes with polymeric substratesImmobilized enzymesKinetics of transport processesEnzyme reactions at membrane interfacesPART III: MethodsMethods for investigating multiple equilibriaElectrochemical methodsCalorimetrySpectroscopic methodsMeasurement of fast reactions §02§Hans Bisswanger was Professor at the Interfaculty Institute of Biochemistry at the University of Tübingen (Germany), where he has developed and taught for many years an intensive course on enzyme kinetics, enzyme technology and ligand binding. His scientific interest lies with structural and regulatory mechanisms of multi-enzyme complexes, thermophilic enzymes and the technical application of immobilized enzymes. He is the author of two well-known books on enzymology that have appeared in different languages and editions.
